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Volume 15 - 2013


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1-7

The significant aberrant expression of FOXC1 as a high specific and sensitive potential biomarker in gastric adenocarcinoma tumor tissues

Author(s): Dr. Mohammadreza, Hajjari,

Abstract: FOX transcription factors regulate a wide array of biological processes including development, differentiation, and invasion. It seems that some FOX proteins are involved in epithelial-to-mesenchymal transition (EMT), which is involved in the progression of cancer. However, the role of FOXC1, one of the members of FOX proteins, remains unknown in the progression of gastric adenocarcinoma. Here, by quantitative Real time PCR, we report the significant up-regulation of FOXC1 in gastric tumors compared with normal tissues. Also, because it was previously reported that FOXC1 expression is correlated with SUZ12 in breast cancer tissues, we wondered if this correlation exists in gastric cancer tissues. Concerning the role of SUZ12, one of the components of Polycomb repressive Complex 2, unraveling the relation between FOXC1 and SUZ12 may help us to decipher the mechanisms of gastric adenocarcinoma. In the current study, we demonstrate that FOXC1 expression can be considered as a good putative biomarker for gastric adenocarcinoma tissues by ROC curve analysis. Based on our results, FOXC1 may potentially be useful as a biomarker or molecular target in treatment or prevention of gastric cancer.

Keywords: FOX transcription factors, EMT, Gastric Cancer, Quantitative Real time-PCR



8-13

Intrathecal injection: An effective technique to implement gene transfection inside central nervous system

Author(s): Dr. Wei-Qing , Kong,

Abstract: Spinal cord injury (SCI) will lead to functional deficit of limbs and sometimes even affect the patient’s life. Now, little measures are effective except administration of great dosage of methylprednisolone and decompression surgery in early stage. But general application of the drug may cause side effects and operation can only remove the mass that compress the dura or spinal cord. More safe and effective methods will be needed during the operation. We use intrathecal injection to implement gene transfection inside central nervous system (CNS). Through gene delivery technique, continuous expression of functional protein, such as neuro-protective factor at local site may be possible. In mRNA level, expression of reporter gene was demonstrated at the 2-week point by display of Lac Z-mRNA via RT-PCR. However, no bands of control group can be detected by this way except β-actin. Experiment group represents blue dyed cell (neuron or glial cell) in spinal cord, but control group doesn’t. The tissue proximal to injection point represents more positive cells than that of distal to the point (according to quantity analysis). The intrathecal injection technique applied in the current study make regional gene transfection within CNS system possible.

Keywords: Transfection. Spinal cord injury (SCI). In vivo; Gene therapy. Central nervous system (CNS). Intrathecal injection



14-29

Green factories: plastids for the production of foreign proteins at high levels

Author(s): Dr. Niaz Ahmad,

Abstract: The soaring costs along with the increasing demand of recombinant proteins in different walks of life have fuelled the quest to find out alternate modes for their production, which are cheaper, safer and can deliver the modern safety standards. In this context, plants are emerging as an alternative production platform for foreign proteins. Chloroplasts – the green plastids with an in-built remarkable capacity to express foreign proteins at high levels – offer several attractive features, which make plants an exceptionally useful system for low-cost production of high- value targets at large scale. Transformation of chloroplasts, therefore, holds a great potential to meet the challenges in the areas of food, feed and medicine posed by a population on the rise. Numerous developments have been made in the field, all of which set plastids to become a centrepoint of future plant engineering efforts. The present review briefly describes ‘the state of the art’ of the technology along with its salient features whilst highlighting the latest trends in the area of chloroplast transformation.

Keywords: Chloroplast transformation, recombinant proteins, biopharming



30-37

Association of Interleukin 1B -511C/T polymorphism with Osteoarthritis, Juvenile Idiopathic Arthritis and Rheumatoid Arthritis susceptibility: a meta-analysis

Author(s): Dr. Wenru Tang,

Abstract: Background: A number of studies have been conducted to investigate the different types of inflammatory arthritis potential association with the IL-1B -511C/T polymorphism. However, results were inconsistent. We performed this meta-analysis to estimate the association between -511C/T polymorphism and osteoarthritis (OA), juvenile idiopathic arthritis (JIA) and rheumatoid arthritis (RA). Methods: Electronic search of PubMed and CNKI was conducted to select studies. Studies containing available genotype frequencies of -511C/T were chosen, and pooled odds ratio (OR) with 95% confidence interval (CI) was used to assess the association. Results: The final meta-analysis included 17 published studies including 1447 OA cases and 1171 controls, 234 JIA cases and 313 controls, 3524 RA cases and 3111 controls separately. The results suggested that the variant genotype was associated with RA in the total population (additive model: OR 0.925, 95% CI (0.860~0.995), P = 0.035). However, the association was not significant between this polymorphism and OA (additive model: OR=1.067, 95% CI: 0.937~1.214, p= 0.328; dominant model: OR =0.930, 95% CI: 0.706~1.225, p=0.606) or JIA in the total population (additive model: OR=1.067, 95% CI: 0.821~1.338, p= 0.626; dominant model: OR =1.269, 95% CI: 0.843~1.909, p=0.254), The stratification by ethnicity failed to identify any association between this polymorphism and OA, JIA, RA in Asian, Caucasian or African groups. Conclusion: This meta-analysis suggests that the allele T of IL-1B -511C/T polymorphism is associated with decreased risk of RA in all ethnicities including Caucasian, Asian and African.

Keywords: IL-1B-511C/T, meta-analysis, OA, JIA, RA



38-51

Analysis and assessment of radiological findings from a Phase II study of liposomal cisplatin (LipoplatinTM) plus gemcitabine versus cisplatin plus gemcitabine as first line treatment in inoperable non-small cell lung cancer

Author(s): Dr. Faye Lazarioti,

Abstract: Lung cancer is the most common cause of cancer and responsible for 28% of all deaths associated with cancer. One of the cytotoxic agents most commonly used in the treatment of NSCLC is cisplatin. However, the clinical use of cisplatin is associated with significant toxicities. LipoplatinTM is a new liposomal cisplatin, designed to reduce cisplatin toxicities without reduce efficacy. Imaging is a key component in the assessment of response to lung cancer therapy and is a determinant of the therapeutic decisions in cancer patients. The purpose of this article is the analysis and assessment of radiological findings, which is mainly based the evaluation of response to chemotherapy and there correlation with the histological subtypes in a phase II prospective, randomized, comparative, open label study and in addition compare the efficacy and safety of Lipoplatin-gemcitabine versus cisplatin-gemcitabine combination as first line treatment in advanced NSCLC.

Keywords: NSCLC, Computed Tomography, DPPG, cisplatin, RECIST, Lipoplatin




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