80% cytotoxicity, determined by the Alamar Blue assay. In contrast, less than 5% of H357, H413 and H376 cells was positive for β-gal staining. Nevertheless, in H357 cells about 80% and 50% cytotoxicity were observed with Fugene and Escort+TF, respectively. In H413 cells, the viability was 40% with Fugene and 20% with Escort+TF, while in H376 cells it was reduced to 60% only with Fugene. Thus, cytotoxicity in the presence of GCV was observed despite the very low efficiency of transfection, in these cell lines. Lipid-DNA complexes may be useful for suicide gene therapy of oral squamous cell carcinoma (OSCC) cells since a high percentage of GCV-specific cell death can be achieved despite the low efficiency of transfection, possibly due to the “bystander effect”, and since these non-viral vectors have a favorable safety profile.">
The Publication: Gene transfer to human oral cancer cells via non- viral vectors and HSV-tk/ganciclovir-mediated
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